Kinetics of actin-myosin binding. II. Two-variable model and actin gelation
نویسندگان
چکیده
منابع مشابه
F-actin and Myosin II Binding Domains in Supervillin*□S
Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterolrich domains called lipid rafts. A subset of these detergent-resistant membranes (DRM-H) exhibits a higher buoyant density ( 1.16 g/ml) because of association with membrane skeleton proteins, including actin, myosin II, myosin 1G, fodrin, and an actinand membrane-binding protein called superv...
متن کاملF-actin and myosin II binding domains in supervillin.
Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts. A subset of these detergent-resistant membranes (DRM-H) exhibits a higher buoyant density ( approximately 1.16 g/ml) because of association with membrane skeleton proteins, including actin, myosin II, myosin 1G, fodrin, and an actin- and membrane-binding prot...
متن کاملActin assembly factors regulate the gelation kinetics and architecture of F-actin networks.
Dynamic regulation of the actin cytoskeleton is required for diverse cellular processes. Proteins regulating the assembly kinetics of the cytoskeletal biopolymer F-actin are known to impact the architecture of actin cytoskeletal networks in vivo, but the underlying mechanisms are not well understood. Here, we demonstrate that changes to actin assembly kinetics with physiologically relevant prot...
متن کاملBinding of myosin A to F-actin.
The studies of Szent-Gyorgyi (1) have clearly established the formation of actomyosin from F-actin and myosin A. SzentGyorgyi (l), Portzehl, Schramm, and Weber (2), Jaisle (3), Snellman and Gelotte (4), and Spicer and Gergely (5) have suggested from their viscometric and centrifugal studies that the maximal amount of myosin A which can be bound by F-actin is in the ratio of 2 to 4 : 1, by weigh...
متن کاملKinetics of binding of caldesmon to actin.
The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan actin. The concentration dependence of the observed rate of caldesmon-actin binding was analyzed to a first approximation as a single-step reaction using a Monte Carlo simulat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1987
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(87)83330-1